Article
A modified pulsed-field gel electrophoresis (PFGE) protocol for subtyping previously non-PFGE typeable isolates of Clostridium difficile polymerase chain reaction ribotype 001.
The Journal of hospital infection - 1 Nov 2005
Gal M, Northey G, Brazier J S
Abstract excerpt
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to 50 isolates of the UK epidemic strain of Clostridium difficile, polymerase chain reaction (PCR) ribotype 001, to develop a PFGE-based subtyping scheme. This protocol overcame the inherent DNA degradation prob...
Topics
- Bacterial Typing Techniques
- Clostridioides difficile
- Cluster Analysis
- DNA Fingerprinting
- DNA, Bacterial
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Gel, Pulsed-Field
- Enterocolitis, Pseudomembranous
- Genotype
- Humans
- Molecular Epidemiology
- Ribotyping
- United Kingdom