Article
Dynamic expansion culture for mesenchymal stem cells.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Majd Hicham, Quinn Thomas M, Wipff Pierre-Jean, Hinz Boris
Abstract excerpt
To be applied in sufficient numbers for regenerative medicine, primary mesenchymal stem cells (MSCs) need to be amplified in culture. Standard cell culture involves regular passing because MSC proliferation in size-limited culture vessels stagnates due to contact inhibition of growth. The use of harmful enzymes for passaging and the mechanical properties of standard culture vessels change the MSC phenotype....
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