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Article

[Development of an ultrasensitive mismatch tolerant PCR assay for the qualitative and quantitative determination of HIV-1 RNA].

Voprosy virusologii - 1 Jan 2000

Prasolova M A, Ivanov M K, Dymshits G M

Abstract excerpt

A real-time fluorescent polymerase chain reaction was used to develop a RealBest HIV RNA kit that was clinically suitable for the detection of HIV-1 RNA and for the estimation of virus load in plasma and serum samples. Due to the selection of a highly conserved target region and to the experimental study of the impact of different primer-template and probe-template mismatches on RT-PCR with subsequent selection...

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Identifiers

PMID: 21427951

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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