Article
A non-radioactive multiprime sequencing method for HIV genomes.
Journal of virological methods - 1 Feb 1995
Huber J, Hell W, Wolf H
Abstract excerpt
A manual non-radioactive DNA sequencing protocol was developed for rapid analysis of variable HIV-1 genomes. Sets of up to ten primers were used in one sequencing reaction. After polyacrylamide gel electrophoresis and blotting onto nylon membranes the individual sequences were detected by hybridi...
Topics
- DNA Primers
- DNA Probes
- Genetic Variation
- Genome, Viral
- HIV-1
- Humans
- Luminescent Measurements
- Molecular Probe Techniques
- Sequence Analysis, DNA