Article
Isolation of copper biochelates from Methylosinus trichosporium OB3b and soluble methane monooxygenase mutants.
Applied and environmental microbiology - 1 Mar 1998
Téllez C M, Gaus K P, Graham D W, Arnold R G, Guzman R Z
Abstract excerpt
Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exclusion high-performance liquid chromatography. A single chromatographic peak, when present,...
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