Article
Each yeast mitochondrial F1F0-ATP synthase complex contains a single copy of subunit 8.
Biochimica et biophysica acta - 8 Dec 2003
Stephens Andrew N, Nagley Phillip, Devenish Rodney J
Abstract excerpt
The stoichiometry of subunit 8 in yeast mitochondrial F(1)F(0)-ATP synthase (mtATPase) has been evaluated using an immunoprecipitation approach. Single HA or FLAG epitopes were introduced at the N-terminus of subunit 8. Expression of each tagged subunit 8 variant in yeast cells lacking endogenous subunit 8 restored a respiratory phenotype and had little measurable effect on ATP hydrolase activity of the isolated...
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