Article
[Glycosylation and L303e/F309S mutations improve intein-mediated splicing of the split coagulation factor VIII].
Yao xue xue bao = Acta pharmaceutica Sinica - 1 Nov 2010
Zhu Fu-Xiang, Liu Ze-Long, Miao Jing, Qu Hui-Ge, Chi Xiao-Yan
Abstract excerpt
We recently demonstrated that an intein-mediated protein splicing can be used to transfer B-domain-deleted FVIII (BDD-FVIII) gene by a dual-vector. In this study, we observed the effect of a variant heavy chain with six potential glycosylation sites of B domain and L303E/F309S mutations in its A1 domain, which were proven to be beneficial for FVIII secretion, on secretion of spliced BDD-FVIII. By transient...
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