A novel vector for a rapid generation of fiber-mutant adenovirus based on one step ligation and quick screening of positive clones.

Abstract excerpt

The generation of fiber-modified adenoviral vector has proven difficult. In the paper, we developed a new system for rapid construction of fiber-modified adenoviral vector containing foreign peptides in the HI loop or C-terminal of the fiber knob. The new system was established through the following processes. First, a unique BamHI mutation was made in the genome of Ad5 without causing amino acid change. Second,...

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