Article
Inhibition of heat tolerance and nuclear import of Gts1p by Ssa1p and Ssa2p.
Bioscience, biotechnology, and biochemistry - 1 Jan 2011
Sanada Mitsuaki, Kuroda Kouichi, Ueda Mitsuyoshi
Abstract excerpt
The Saccharomyces cerevisiae gene GTS1 is pleiotropic. GTS1 induction produces a variety of biological phenomena represented by heat tolerance. To clarify the interaction partners of Gts1p, tandem affinity purification and immunoprecipitation were performed. Ssa1p and Ssa2p, members of the 70-kDa heat-shock protein family, were identified. Co-expression of SSA1 or SSA2 inhibited Gts1p nuclear import. As compared...
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