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Tyrosine 656 in topoisomerase IIβ is important for the catalytic activity of the enzyme: Identification based on artifactual +80-Da modification at this site.

Proteomics - 1 Mar 2011

Grozav Adrian G, Willard Belinda B, Kozuki Toshiyuki, Chikamori Kenichi, Micluta Marius A, Petrescu Andrei-Jose, Kinter Michael, Ganapathi Ram, Ganapathi Mahrukh K

Abstract excerpt

Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +80-Da modified sites: tyr656, ser1395, thr1426 and ser1545. Phosphorylation at ser1395, thr1426...

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Identifiers

PMID: 21280220
DOI: 10.1002/pmic.201000194
PMCID: PMC3608106

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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