Article
Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
Journal of bacteriology - 1 Apr 2011
Eckert Sabine E, Dziva Francis, Chaudhuri Roy R, Langridge Gemma C, Turner Daniel J, Pickard Derek J, Maskell Duncan J, Thomson Nicholas R, Stevens Mark P
Abstract excerpt
Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
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