Article
Model SNP development for complex genomes based on hexaploid oat using high-throughput 454 sequencing technology.
BMC genomics - 27 Jan 2011
Oliver Rebekah E, Lazo Gerard R, Lutz Joseph D, Rubenfield Marc J, Tinker Nicholas A, Anderson Joseph M, Wisniewski Morehead Nicole H, Adhikary Dinesh, Jellen Eric N, Maughan P Jeffrey, Brown Guedira Gina L, Chao Shiaoman, Beattie Aaron D, Carson Martin L, Rines Howard W, Obert Donald E, Bonman J Michael, Jackson Eric W
Abstract excerpt
BACKGROUND: Genetic markers are pivotal to modern genomics research; however, discovery and genotyping of molecular markers in oat has been hindered by the size and complexity of the genome, and by a scarcity of sequence data. The purpose of this study was to generate oat expressed sequence tag (EST) information, develop a bioinformatics pipeline for SNP discovery, and establish a method for rapid,...
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