Article
A targeted genotyping-by-sequencing tool (Rapture) for genomics-assisted breeding in oat.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Feb 2020
Bekele Wubishet A, Itaya Asuka, Boyle Brian, Yan Weikai, Mitchell Fetch Jennifer, Tinker Nicholas A
Abstract excerpt
We adapted and tested a Rapture assay as an enhancement of genotyping-by-sequencing (GBS) in oat (Avena sativa). This assay was based on an additional bait-based capture of specific DNA fragments representing approximately 10,000 loci within the enzyme-based complexity reduction provided by GBS. By increasing the specificity of GBS to include only those fragments that provided effective polymorphic markers, it...
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