Article
Development of a genome-wide marker design workflow for onions and its application in target amplicon sequencing-based genotyping.
DNA research : an international journal for rapid publication of reports on genes and genomes - 23 Aug 2022
Sekine Daisuke, Oku Satoshi, Nunome Tsukasa, Hirakawa Hideki, Tsujimura Mai, Terachi Toru, Toyoda Atsushi, Shigyo Masayoshi, Sato Shusei, Tsukazaki Hikaru
Abstract excerpt
Onions are one of the most widely cultivated vegetables worldwide; however, the development and utilization of molecular markers have been limited because of the large genome of this plant. We present a genome-wide marker design workflow for onions and its application in a high-throughput genotyping method based on target amplicon sequencing. The efficiency of the method was evaluated by genotyping of F2...
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