Article
Multimerization-defective variants of dodecameric secretin PulD.
Research in microbiology - 1 Jan 2000
Guilvout Ingrid, Nickerson Nicholas N, Chami Mohamed, Pugsley Anthony P
Abstract excerpt
The C-terminal core domain of the secretin PulD from Klebsiella oxytoca forms heat-resistant dodecameric complexes within less than 10min in an Escherichia coli in vitro transcription-translation system containing liposomes, and is toxic when made in the cytoplasm without a signal peptide. Random mutagenesis of DNA encoding this region of PulD revealed that amino acid changes throughout almost its entire length...
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