Article
Characteristics of intracellular proteolytic activities of Bacillus megaterium.
Folia microbiologica - 1 Jan 1990
Moravcová J, Chaloupka J
Abstract excerpt
Intracellular proteolytic activities of B. megaterium KM occur soluble in the cytoplasm and periplasm and insoluble in the membrane. Two proteolytic enzymes were found in the cytoplasmic fraction by gel filtration on Sephadex G 150 and by polyacrylamide gel electrophoresis. The first enzyme called CI was stable, had a relative molecular mass of Mr = 105,000 (M = 105 kg/mol) and was inhibited by EDTA and PMSF,...
Topics
- Bacillus megaterium
- Chromatography, Gel
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Mutation