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Article

Force spectroscopy of substrate molecules en route to the proteasome's active sites.

Biophysical journal - 19 Jan 2011

Classen Mirjam, Breuer Sarah, Baumeister Wolfgang, Guckenberger Reinhard, Witt Susanne

Abstract excerpt

We used an atomic force microscope to study the mechanism underlying the translocation of substrate molecules inside the proteasome. Our specific experimental setup allowed us to measure interaction forces between the 20S proteasome and its substrates. The substrate (β-casein) was covalently boun...

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Identifiers

PMID: 21244845
DOI: 10.1016/j.bpj.2010.12.3689
PMCID: PMC3021671

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-21244845-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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