Article
A new PCR-SSP method for HLA DR-DQ risk assessment for celiac disease.
Clinica chimica acta; international journal of clinical chemistry - 11 Apr 2011
Lavant Ewa H, Agardh Daniel J, Nilsson Anita, Carlson Joyce A
Abstract excerpt
BACKGROUND: Susceptibility to celiac disease is essentially restricted to carriers of specific HLA DQA1 and DQB1 alleles. We have developed a semi-automated sequence specific primer (SSP) PCR method for clinical HLA typing and compared the test results with those from a commercial method. METHODS: Primers for each DQA1 and DQB1 allele group were included in our PCR-SSP reaction to allow differentiation of...
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