Resonance Raman study on mutant cytochrome P-450 obtained by site-directed mutagenesis.

doi:10.1016/0167-4838(90)90078-t

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Article

Resonance Raman study on mutant cytochrome P-450 obtained by site-directed mutagenesis.

Biochimica et biophysica acta - 3 Sept 1990

Egawa T, Imai Y, Ogura T, Kitagawa T

Abstract excerpt

Resonance Raman spectra were observed for the threonine-301 to serine or valine mutant as well as the wild type of rabbit liver microsomal cytochrome P-450 [laurate(omega-1)-hydroxylase] (P-450(omega-1], which were prepared through site-directed mutagenesis. The high-spin marker resonance Raman (...

Topics

Animals
Cytochrome P-450 CYP4A
Cytochrome P-450 Enzyme System
Microsomes, Liver
Mixed Function Oxygenases
Mutation
Oxidation-Reduction
Protein Conformation
Rabbits
Saccharomyces cerevisiae
Serine
Spectrum Analysis, Raman
Threonine
Valine

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