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Structural and functional characterization of "laboratory evolved" cytochrome P450cam mutants showing enhanced naphthalene oxygenation activity.

Biochemical and biophysical research communications - 29 Oct 2004

Matsuura Koji, Tosha Takehiko, Yoshioka Shiro, Takahashi Satoshi, Ishimori Koichiro, Morishima Isao

Abstract excerpt

To elucidate molecular mechanisms for the enhanced oxygenation activity in the three mutants of cytochrome P450cam screened by 'laboratory evolution' [Nature 399 (1999) 670], we purified the mutants and characterized their functional and structural properties. The electronic absorption and resonance Raman spectra revealed that the structures of heme binding site of all purified mutants were quite similar to that...

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PMID: 15451425
DOI: 10.1016/j.bbrc.2004.08.221

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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