Article
Mutational analysis of wheat (Triticum aestivum L.) nucleotide pyrophosphatase/phosphodiesterase shows the role of six amino acids in the catalytic mechanism.
Applied microbiology and biotechnology - 1 Apr 2011
Joye Iris J, Beliën Tim, Brijs Kristof, Soetaert Wim, Delcour Jan A
Abstract excerpt
Nucleotide pyrophosphatases/phosphodiesterases (NPPs, PF01663) release nucleoside 5'-monophosphates from a wide range of nucleotide substrates. Only very recently, the first plant members of the NPP family were characterised (Joye et al. J Cereal Sci 51: 326-336, 2010), and little is known about their substrate-specifying residues. We elucidated the role of six amino acid residues of the recently identified and...
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