Article
Holin triggering in real time.
Proceedings of the National Academy of Sciences of the United States of America - 11 Jan 2011
White Rebecca, Chiba Shinobu, Pang Ting, Dewey Jill S, Savva Christos G, Holzenburg Andreas, Pogliano Kit, Young Ry
Abstract excerpt
During λ infections, the holin S105 accumulates harmlessly in the membrane until, at an allele-specific time, suddenly triggering to form irregular holes of unprecedented size (>300 nm), releasing the endolysin from the cytoplasm, resulting in lysis within seconds. Here we used a functional S105-GFP chimera and real-time deconvolution fluorescence microscopy to show that the S105-GFP fusion accumulated in a...
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