Article
Construction and properties of a temperature-sensitive mutation in the gene for the bacteriophage SPO1 DNA-binding protein TF1.
Journal of bacteriology - 1 Aug 1990
Sayre M H, Geiduschek E P
Abstract excerpt
The Bacillus subtilis bacteriophage SPO1 encodes the DNA-binding protein TF1, a homolog of the ubiquitous type II DNA-binding proteins that are components of bacterial chromatin. The known three-dimensional structure of a related protein was used in devising a scheme of site-directed mutagenesis that led to the creation of a temperature-sensitive mutation in the TF1 gene. At the nonpermissive temperature, this...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Bacteriophages
- Base Sequence
- DNA Replication
- DNA, Viral
- DNA-Binding Proteins
- Genes, Viral
- Geobacillus stearothermophilus
- Kinetics
- Models, Molecular