Article
Mechanisms of 2'-deoxyguanosine toxicity in mouse T-lymphoma cells with purine nucleoside phosphorylase deficiency and resistance to inhibition of ribonucleotide reductase by dGTP.
The Biochemical journal - 15 Jun 1990
Duan D S, Nagashima T, Hoshino T, Waldman F, Pawlak K, Sadee W
Abstract excerpt
Purine nucleoside phosphorylase (PNP; EC 2.4.2.1) deficiency is thought to cause T-lymphocyte depletion by accumulation of dG and dGTP, resulting in feedback inhibition of ribonucleotide reductase (RR; EC 1.17.4.1) and hence DNA synthesis. To test for additional toxic mechanisms of dG, we selected a double mutant of the mouse T-lymphoma S-49 cell line, dGuo-L, which is deficient in PNP and partially resistant to...
Topics
- Animals
- Cell Cycle
- Cell Division
- DNA, Neoplasm
- Deoxyguanine Nucleotides
- Deoxyguanosine
- Lymphoma
- Mice
- Mutation
- Pentosyltransferases
- Purine-Nucleoside Phosphorylase