Article
[Unusual structure of the regulatory region of the riboflavin biosynthesis operon in Bacillus subtilis].
Molekuliarnaia biologiia - 1 Jan 2000
Mironov V N, Perumov D A, Kraev A S, Stepanov A I, Skriabin K G
Abstract excerpt
In vitro mutagenesis with methylhydroxylamine and nitrosomethylurea was used to obtain a number of Bacillus subtilis mutants impaired in flavin-dependent response. Mutants displayed varying degree of flavin-dependent repression of riboflavin synthase and of 6,7-dimethyl-8-ribityl-lumasine accumulation. Single nucleotide substitutions were detected by DNA sequencing in all of the mutants, affecting the 48 b.p....
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