Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

BsaXI/RFLP analysis of initial or selectively reamplified PCR product is unreliable in detecting the V617F mutation in JAK2.

International journal of laboratory hematology - 1 Jun 2011

Shepard G C, Lawson H L, Hawkins G A, Owen J

Abstract excerpt

INTRODUCTION: Laboratory testing for the presence of the V617F mutation in JAK2 has taken on great importance in the diagnosis of myeloproliferative disorders. The availability of a facile detection method would bring this testing into greater clinical use. The polymerase chain reaction coupled with restriction fragment length polymorphisms is such a facile method. BsaXI cleaves the normal sequence but does not...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to JAK2 gene
Share to Janus Kinase 2
Share to Mutation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Myeloproliferative Disorders

Share to Polymerase Chain Reaction

Share to Polymorphism, Restriction Fragment Length

Share to Sequence Analysis, DNA

Source

Identifiers

PMID: 21118387
DOI: 10.1111/j.1751-553X.2010.01282.x

Genes

JAK2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

BsaXI/RFLP analysis of initial or selectively reamplified PCR product is unreliable in detecting the V617F mutation in JAK2.PMID 21118387Read ↗

Development of a highly sensitive method for detection of JAK2V617F.Semantic proximity Read ↗

Sensitive detection and quantification of the JAK2V617F allele by real-time PCR blocking wild-type amplification by using a peptide nucleic acid oligonucleotide.Semantic proximity Read ↗

Two-round allele specific-polymerase chain reaction: a simple and highly sensitive method for JAK2V617F mutation detection.Semantic proximity Read ↗

Preferential PCR amplification of alleles: mechanisms and solutions.Semantic proximity Read ↗

Development of a reliable PCR-RFLP assay for investigation of the JAK2 rs10974944 SNP, which might predispose to the acquisition of somatic mutation JAK2(V617F).Semantic proximity Read ↗

Molecular diagnosis of the myeloproliferative neoplasms: UK guidelines for the detection of JAK2 V617F and other relevant mutations.Semantic proximity Read ↗

Comparative study of different methodologies to detect the JAK2 V617F mutation in chronic BCR-ABL1 negative myeloproliferative neoplasmsSemantic proximity Read ↗

Highly Sensitive Blocker Displacement Amplification for Detection of Low-Level JAK2V617F Variant.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.