Article
Use of a Real-Time PCR TaqMan Assay for Rapid Identification and Differentiation of <i>Burkholderia pseudomallei</i> and <i>Burkholderia mallei</i>
1 Nov 2005
Abstract excerpt
A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.
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