Article
Cloning and characterization of Kluyveromyces marxianus Hog1 gene.
Biotechnology letters - 1 Mar 2011
Qian Jiangchao, Qin Xiulin, Yin Qin, Chu Ju, Wang Yonghong
Abstract excerpt
The mitogen-activated protein kinase Hog1 gene (Kmhog1) was isolated from Kluyveromyces marxianus strain NBRC 1777 by degenerate PCR and genome walking, and then disrupted to construct a mutant strain hog1∆. The mutant was now more sensitive to acetic acid and its growth was nearly completely inhibited by 0.5 M NaCl (97%) and 10 mM H(2)O(2) (93%) as compared with the wild-type cells. However, neither strain grew...
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