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Article

Microchannel-nanopore device for bacterial chemotaxis assays.

Analytical chemistry - 15 Nov 2010

Kovarik Michelle L, Brown Pamela J B, Kysela David T, Berne Cécile, Kinsella Anna C, Brun Yves V, Jacobson Stephen C

Abstract excerpt

Motile bacteria bias the random walk of their motion in response to chemical gradients by the process termed chemotaxis, which allows cells to accumulate in favorable environments and disperse from less favorable ones. In this work, we describe a simple microchannel-nanopore device that establishes a stable chemical gradient for chemotaxis assays in ≤1 min. Chemoattractant is dispensed by diffusion through 10 nm...

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Identifiers

PMID: 20961116
DOI: 10.1021/ac101977f
PMCID: PMC2992552

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