Article
[Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin I].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Feb 2010
Shen Hong-Hui, Hou Jun, Hu Yan, Bai Bing-Ke, Wang Zhi-Jie, Yu Xiang-Hui, Kong Wei, Liu Ze, Mao Pan-Yong
Abstract excerpt
OBJECTIVE: To develop a vector inserted with complete genome of poliovirus strain Sabin I. METHODS: The 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified. RESULTS: The complete genome of poliovirus strain Sabin I was successfully...
Topics
- Cloning, Molecular
- Genetic Vectors
- Genome, Viral
- Mutation
- Poliovirus
