Article
Time-saving multiplex detection of single nucleotide polymorphisms by ultrasensitive DNA microarray.
Journal of biochemistry - 1 Nov 2010
Ichikawa Makiko, Miwa Keishi, Yamasaki Tomo, Nakagawa Izumi, Takizawa Satoko, Masuda Satohiro, Inui Ken-ichi
Abstract excerpt
Rapid and multiplex detection system using an ultrasensitive DNA microarray was developed and utilized for the analysis of six pharmacokinetically relevant single nucleotide polymorphisms (SNPs) (MDR1-C1236T, MDR1-G2677TA, MDR1-C3435T, CYP3A5-A6986G, CYP2C19-G681A, CYP2C19-G636A) from blood samples derived from liver transplant patients. The SNP detection system is comprised of three processes: multiplex PCR,...
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