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Article

Identification and characterization of a putative dihydroorotase, KPN01074, from Klebsiella pneumoniae.

The protein journal - 1 Aug 2010

Wang Chuan-Cheng, Tsau Huai-Wen, Chen Wei-Ti, Huang Cheng-Yang

Abstract excerpt

Dihydroorotase (DHO; EC 3.5.2.3) is an essential metalloenzyme in the biosynthesis of pyrimidine nucleotides. Here, we identified and characterized DHO from the pathogenic bacterium Klebsiella pneumoniae (Kp). The activity of KpDHO toward L: -dihydroorotate was observed with K (m) = 0.04 mM and V (max) = 8.87 mumol/(mg min). Supplementing the standard growth medium with Co2+, Mn2+, Mg2+, or Ni2+ increased enzyme...

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Identifiers

PMID: 20676924
DOI: 10.1007/s10930-010-9272-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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