When technology meets conservation: increased microsatellite marker production using 454 genome sequencing on the endangered Okaloosa Darter (Etheostoma okaloosae).

Abstract excerpt

High-throughput sequencing affords a cost and time effective means of obtaining large numbers of genetic markers for conservation studies. Here, we present thousands of novel microsatellite loci developed for the Okaloosa darter, Etheostoma okaloosae, an endangered percid fish. We sequenced more than 29 million bp using 454 whole genome shotgun sequencing and employed free user-friendly bioinformatics tools to...

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