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Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing.

Genetics and molecular research : GMR - 20 Jun 2016

Gichira A W, Long Z C, Hu G W, Gituru R W, Wang Q F, Chen J M

Abstract excerpt

Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individuals. The average number of alleles per locus was 7.6, while...

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PMID: 27420948
DOI: 10.4238/gmr.15028168

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