A hypervariable region of P450IIC5 confers progesterone 21-hydroxylase activity to P450IIC1.

doi:10.1021/bi00239a003

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Article

A hypervariable region of P450IIC5 confers progesterone 21-hydroxylase activity to P450IIC1.

Biochemistry - 25 Jun 1991

Kronbach T, Kemper B, Johnson E F

Abstract excerpt

Cytochrome P450IIC5 is a hepatic progesterone 21-hydroxylase while the 95% identical P450IIC4 has a greater than 10-fold higher Km for progesterone 21-hydroxylation and the 74% identical P450IIC1 does not hydroxylate progesterone at detectable rates. Previous work demonstrated that the apparent Km of P450IIC4 for progesterone 21-hydroxylation can be markedly improved by replacing a valine at position 113 with an...

Topics

Amino Acid Sequence
Animals
Base Sequence
Camphor 5-Monooxygenase
Cell Line
Cytochrome P-450 Enzyme System
Cytochrome P450 Family 2
DNA
Genetic Variation
Hydroxylation
Microsomes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mixed Function Oxygenases

Molecular Sequence Data

Mutagenesis, Site-Directed

Steroid 21-Hydroxylase

Substrate Specificity

Transfection

Source

Open in PubMed

Identifiers

PMID: 2059619
DOI: 10.1021/bi00239a003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2059619-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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