Article
Phenotypic annotation of the mouse X chromosome.
Genome research - 1 Aug 2010
Cox Brian J, Vollmer Marion, Tamplin Owen, Lu Mei, Biechele Steffen, Gertsenstein Marina, van Campenhout Claude, Floss Thomas, Kühn Ralf, Wurst Wolfgang, Lickert Heiko, Rossant Janet
Abstract excerpt
Mutational screens are an effective means used in the functional annotation of a genome. We present a method for a mutational screen of the mouse X chromosome using gene trap technologies. This method has the potential to screen all of the genes on the X chromosome without establishing mutant animals, as all gene-trapped embryonic stem (ES) cell lines are hemizygous null for mutations on the X chromosome. Based...
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