Article
Septal and lateral wall localization of PBP5, the major D,D-carboxypeptidase of Escherichia coli, requires substrate recognition and membrane attachment.
Molecular microbiology - 1 Jul 2010
Potluri Lakshmiprasad, Karczmarek Aneta, Verheul Jolanda, Piette Andre, Wilkin Jean-Marc, Werth Nadine, Banzhaf Manuel, Vollmer Waldemar, Young Kevin D, Nguyen-Distèche Martine, den Blaauwen Tanneke
Abstract excerpt
The distribution of PBP5, the major D,D-carboxypeptidase in Escherichia coli, was mapped by immunolabelling and by visualization of GFP fusion proteins in wild-type cells and in mutants lacking one or more D,D-carboxypeptidases. In addition to being scattered around the lateral envelope, PBP5 was also concentrated at nascent division sites prior to visible constriction. Inhibiting PBP2 activity (which eliminates...
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