Article
Strikingly fast microtubule sliding in bundles formed by Chlamydomonas axonemal dynein.
Cytoskeleton (Hoboken, N.J.) - 1 Jun 2010
Aoyama Susumu, Kamiya Ritsu
Abstract excerpt
Chlamydomonas axonemal extracts containing outer-arm dynein bundle microtubules when added in the absence of ATP. The bundles dissociate after addition of ATP (Haimo et al., Proc Natl Acad Sci USA 76:5759-5768, 1979). In the present study, we investigated the ATP-induced bundle dissociation process using caged ATP. Application of approximately 0.5 mM ATP induced microtubule sliding at approximately 30...
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