Article
High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6')-Ib-cr.
Antimicrobial agents and chemotherapy - 1 Aug 2010
Hidalgo-Grass Carlos, Strahilevitz Jacob
Abstract excerpt
We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6')-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6')-Ib and aac(6')-Ib-cr coexist.
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