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Article

Melting curve analysis for rapid detection of topoisomerase gene mutations in Haemophilus influenzae.

Journal of clinical microbiology - 1 Mar 2009

Nakamura Shigeki, Yanagihara Katsunori, Morinaga Yoshitomo, Izumikawa Koichi, Seki Masafumi, Kakeya Hiroshi, Yamamoto Yoshihiro, Kamihira Shimeru, Kohno Shigeru

Abstract excerpt

We established a real-time PCR assay with melting curve analysis to rapidly genotype quinolone resistance-determining regions (QRDRs) of gyrase A and topoisomerase IV genes in Haemophilus influenzae. This assay is a useful tool for the detection of fluoroquinolone resistance and for the early detection of preexisting QRDR mutations.

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Source

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Identifiers

PMID: 19129413
DOI: 10.1128/JCM.01645-08
PMCID: PMC2650933

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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