Article
Creation of a bacterial cell controlled by a chemically synthesized genome.
Science (New York, N.Y.) - 2 Jul 2010
Gibson Daniel G, Glass John I, Lartigue Carole, Noskov Vladimir N, Chuang Ray-Yuan, Algire Mikkel A, Benders Gwynedd A, Montague Michael G, Ma Li, Moodie Monzia M, Merryman Chuck, Vashee Sanjay, Krishnakumar Radha, Assad-Garcia Nacyra, Andrews-Pfannkoch Cynthia, Denisova Evgeniya A, Young Lei, Qi Zhi-Qing, Segall-Shapiro Thomas H, Calvey Christopher H, Parmar Prashanth P, Hutchison Clyde A, Smith Hamilton O, Venter J Craig
Abstract excerpt
We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and...
Topics
- Bacterial Proteins
- Base Sequence
- Bioengineering
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Deletion
- Genes, Bacterial