Stabilization of a reaction intermediate as a catalytic device: definition of the functional role of the flexible loop in triosephosphate isomerase.

doi:10.1021/bi00465a005

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Article

Stabilization of a reaction intermediate as a catalytic device: definition of the functional role of the flexible loop in triosephosphate isomerase.

Biochemistry - 3 Apr 1990

Pompliano D L, Peyman A, Knowles J R

Abstract excerpt

The function of the mobile loop of triosephosphate isomerase has been investigated by deleting four contiguous residues from the part of this loop that interacts directly with the bound substrate. From the crystal structure of the wild-type enzyme, it appears that this excision will not significa...

Topics

Carbohydrate Epimerases
Computer Graphics
Escherichia coli
Kinetics
Mutation
Protein Conformation
Structure-Activity Relationship
Substrate Specificity
Triose-Phosphate Isomerase

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