Article
Mutational analysis of the Escherichia coli DEAD box protein CsdA.
Journal of bacteriology - 1 Apr 2007
Turner Anne-Marie W, Love Cheraton F, Alexander Rebecca W, Jones Pamela G
Abstract excerpt
The Escherichia coli cold shock protein CsdA is a member of the DEAD box family of ATP-dependent RNA helicases, which share a core of nine conserved motifs. The DEAD (Asp-Glu-Ala-Asp) motif for which this family is named has been demonstrated to be essential for ATP hydrolysis. We show here that CsdA exhibits in vitro ATPase and helicase activities in the presence of short RNA duplexes with either 3' or 5'...
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