Article
The 3'-terminal sequence of Bamboo mosaic virus minus-strand RNA interacts with RNA-dependent RNA polymerase and initiates plus-strand RNA synthesis.
Molecular plant pathology - 1 Mar 2010
Chen I-Hsuan, Lin Jen-Wen, Chen Yi-Jing, Wang Zi-Chao, Liang Li-Fang, Meng Menghsiao, Hsu Yau-Heiu, Tsai Ching-Hsiu
Abstract excerpt
A 3'-terminal, 77-nucleotide sequence of Bamboo mosaic virus (BaMV) minus-strand RNA (Ba-77), comprising a 5' stem-loop, a spacer and a 3'-CUUUU sequence, can be used to initiate plus-strand RNA synthesis in vitro. To understand the mechanism of plus-strand RNA synthesis, mutations were introduced in the 5' untranslated region of BaMV RNA, resulting in changes at the 3' end of minus-strand RNA. The results showed...
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