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Article

Identification and functional characterization of regions that can be crosslinked to RNA in the helicase-like domain of BaMV replicase.

Virology - 20 Jun 2009

Han Yu-Tsung, Hsu Yau-Heiu, Lo Chia-Wen, Meng Menghsiao

Abstract excerpt

The helicase-like domain of the Bamboo mosaic virus replicase catalyzes the release of 5'-gamma-phosphate from both ATP and 5'-triphosphated RNA by an identical set of catalytic residues with a presumably larger binding pocket for RNA. In this study, the peptidyl regions involved in RNA binding were mapped by reversible formaldehyde crosslinking and mass spectrometry. Eleven residues within these regions were...

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Identifiers

PMID: 19443005
DOI: 10.1016/j.virol.2009.04.013
PMCID: PMC7134557

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