Article
The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli.
Biochimica et biophysica acta - 1 Dec 2010
Yap Lai Lai, Lin Myat T, Ouyang Hanlin, Samoilova Rimma I, Dikanov Sergei A, Gennis Robert B
Abstract excerpt
Cytochrome bo(3) is the major respiratory oxidase located in the cytoplasmic membrane of Escherichia coli when grown under high oxygen tension. The enzyme catalyzes the 2-electron oxidation of ubiquinol-8 and the 4-electron reduction of dioxygen to water. When solubilized and isolated using dodecylmaltoside, the enzyme contains one equivalent of ubiquinone-8, bound at a high affinity site (Q(H)). The quinone...
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