Article
CryoEM structure of Hsp104 and its mechanistic implication for protein disaggregation.
Proceedings of the National Academy of Sciences of the United States of America - 4 May 2010
Lee Sukyeong, Sielaff Bernhard, Lee Jungsoon, Tsai Francis T F
Abstract excerpt
Hsp104 is a ring-forming AAA+ machine that recognizes both aggregated proteins and prion-fibrils as substrates and, together with the Hsp70 system, remodels substrates in an ATP-dependent manner. Whereas the ability to disaggregate proteins is dependent on the Hsp104 M-domain, the location of the M-domain is controversial and its exact function remains unknown. Here we present cryoEM structures of two Hsp104...
Topics
- Cryoelectron Microscopy
- Enzyme Stability
- Heat-Shock Proteins
- Models, Molecular
- Mutation
- Protein Multimerization
- Protein Structure, Quaternary
- Protein Structure, Tertiary
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins