Article
Conserved distal loop residues in the Hsp104 and ClpB middle domain contact nucleotide-binding domain 2 and enable Hsp70-dependent protein disaggregation.
The Journal of biological chemistry - 10 Jan 2014
Desantis Morgan E, Sweeny Elizabeth A, Snead David, Leung Eunice H, Go Michelle S, Gupta Kushol, Wendler Petra, Shorter James
Abstract excerpt
The homologous hexameric AAA(+) proteins, Hsp104 from yeast and ClpB from bacteria, collaborate with Hsp70 to dissolve disordered protein aggregates but employ distinct mechanisms of intersubunit collaboration. How Hsp104 and ClpB coordinate polypeptide handover with Hsp70 is not understood. Here, we define conserved distal loop residues between middle domain (MD) helix 1 and 2 that are unexpectedly critical for...
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