Article
Rubredoxin mutant A51C unfolding dynamics: a Förster Resonance Energy Transfer study.
Biophysical chemistry - 1 May 2010
Santos Andrea, Duarte Américo G, Fedorov Alexander, Martinho José M G, Moura Isabel
Abstract excerpt
The unfolding dynamics of the rubredoxin mutant A51C (RdA51C) from Desulfovibrio vulgaris (DvRd) was studied on the temperature range from 25 degrees C to 90 degrees C and by incubation at 90 degrees C. By Förster Resonance Energy Transfer (FRET) the donor (D; Trp37) to acceptor (A; 1,5-IAEDANS) distance distribution was probed at several temperatures between 25 degrees C and 90 degrees C, and incubation times at...
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