Article
Strategy for degradomic-peptidomic analysis of human blood plasma.
Journal of proteome research - 7 May 2010
Shen Yufeng, Liu Tao, Tolić Nikola, Petritis Brianne O, Zhao Rui, Moore Ronald J, Purvine Samuel O, Camp David G, Smith Richard D
Abstract excerpt
Herein we describe a strategy for degradomic-peptidomic analyses. The human blood peptidome was isolated through application of AC/SEC, which enriched its components by >300-fold. The isolated peptidome components were separated by long column HRLC providing a peak capacity of approximately 300 for species having MWs of up to 20 kDa. The separated species were identified by the FT MS/MS-UStags sequencing method....
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